Genetic diversity of chicken types reared in Ogun State using carbonic anhydrase polymorphism
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This study was carried out to examine the genetic diversity of different chicken types reared in Ogun State using Carbonic anhydrase. A total of 80 birds comprising 25 boilers, 25 layers and 30 indigenous chickens were purposely selected from Abeokuta North, Ado Odo Otta, Sagamu and Ijebu Imushin locations. Blood samples of 5 mL were drawn from the wing vein of each bird via 2ml hypodermal needle into anticoagulant treated (heparinized) vials. Carbonic anhydrase polymorphism was investigated using cellulose acetate paper electrophoresis. Based on the estimate allele frequencies the population was characterized by their genetic distance Two alleles were reported for each of the population (F and S in carbonic anhydrase). The results obtained showed that, the estimate of heterozygosity was 0.80, 0.92 and 0.60 for layers, boilers and indigenous chicken population respectively. The closest genetic distance relationship was found between the boilers and layers chicken populations (D = 0.0008), as compared to the genetic distance relationship of the indigenous chickens and broilers (D = 0.0459). The relationships among the chicken population were consolidated by the dendrogram that emanated from their genetic distance. The result obtain will be useful as an initial guide in defining objectives for future investigations of genetic integrity and developing conservation strategies for chicken species.